High-performance fluorescence spectrometer capable of sensitive and flexible optical measurements across a wide range of applications, combining advanced optics with a pulsed xenon flash lamp to deliver fast, high-signal-to-noise data for steady-state and kinetic fluorescence studies
- Measurement modes: fluorescence, phosphorescence, chemiluminescence, bioluminescence, and time-resolved phosphorescence.
- Spectral range: 200–900 nm for both excitation and emission.
- Light source: xenon flash lamp delivering high peak intensity with long lifetime.
- Optical system: Czerny-Turner monochromators for excitation and emission with selectable spectral bandwidths (e.g., 1.5–20 nm).
- Detection: red-sensitive photomultiplier tubes (PMTs) for high sensitivity.
- Fast kinetics: rapid scanning (up to ~24 000 nm/min) without wavelength shifts, enabling real-time kinetic experiments.
- Software: intuitive Cary WinFLR software for acquisition, analysis, and advanced plotting (3D, contour).
- Accessories: optional microplate reader, temperature-controlled cuvette holders, polarizers, fiber-optic probes and rapid-mix/stopped flow modules for expanded capabilities.
The Cary Eclipse supports a broad range of research and analytical tasks in both life sciences and physical sciences, including:
- Fluorescence spectroscopy: excitation-emission scans, spectra profiling, quantum yield determination.
- Biochemical assays: ligand binding, protein–nucleic acid interactions, enzyme kinetics, FRET studies.
- Phosphorescence and chemiluminescence: measurements of long-lived emitters and light generated bychemical/biochemical reactions.
- Time-resolved studies: kinetic data collection with high temporal resolution in steady-state and transient modes.
- Temperature-dependent fluorescence: using temperature-controlled accessories to study thermal effects on fluorophores and biomolecules.
