Directly measure the strength of molecular interactions in solution, without the need for immobilization. Characterize nearly all types of molecular interactions with ultra-low sample consumption and versatile capabilities
Monolith X uses Spectral Shift technology to quantify molecular interactions between a target and a ligand.
When a target is labeled with a fluorophore, it gives a particular emission spectrum. If a ligand binds, the fluorophore’s chemical envirnment is changed, causing a blue-shift or re-shift or broadening of the emission peak
To quantify molecular interactions with MST, start by labeling your target molecule with a fluorescent dye and mix it with your ligand. A very precise and brief laser-induced temperature change is applied, which amplifies the variation in fluorescence intensity caused by the ligand binding to your target.
The Kd is calculated by plotting the change in fluorescence against the ligand concentration
Signal measured at two distinctive wavelengths
Ratio of signals plotted against ligand concentration
